Current Protocols in Chemical Biology

Aims & Scope

Sound and reproducible laboratory methods are the foundation of scientific discovery.

Yet all too often, nuances that are responsible for an experiment’s success are not captured in the primary literature, but exist only as part of a lab’s oral tradition.

The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area.

Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables.

Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments.

Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge.

Quarterly issues allow Current Protocols in Chemical Biology to constantly evolve to keep pace with the newest discoveries and developments.

Abstracting & Indexing

Journal is indexed in leading academic databases, ensuring global visibility and accessibility of our peer-reviewed research.

• Scopus
• Google Scholar

Subjects & Keywords

Journal’s research areas, covering key disciplines and specialized sub-topics in Biochemistry, Genetics and Molecular Biology, designed to support cutting-edge academic discovery.

Most Cited Articles

The Most Cited Articles section features the journal's most impactful research, based on citation counts. These articles have been referenced frequently by other researchers, indicating their significant contribution to their respective fields.

• Copper‐Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation

  Citation: 358

  Authors: Stanislav I., Vu Phong, M.G.

• Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single‐cell Imaging

  Citation: 192

  Authors: Jia‐Ren, Mohammad, Jia‐Yun, Peter K.

• Target Identification Using Drug Affinity Responsive Target Stability (DARTS)

  Citation: 187

  Authors: Brett, Gwanghyun, James A., Jing

• Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding

  Citation: 159

  Authors: Nathan J.

• Target Identification by Diazirine Photo‐Cross‐Linking and Click Chemistry

  Citation: 116

  Authors: Andrew L., Jack

• Activity‐Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry

  Citation: 108

  Authors: Anna E., Benjamin F.

• Chemical Synthesis of Hydrocarbon‐Stapled Peptides for Protein Interaction Research and Therapeutic Targeting

  Citation: 82

  Authors: Gregory H., W., Loren D.

• Chemical Genetic Approach for Kinase‐Substrate Mapping by Covalent Capture of Thiophosphopeptides and Analysis by Mass Spectrometry

  Citation: 78

  Authors: Nicholas T., Beatrice T., Jasmina J., Chao, Arvin C., Alma L., Kevan M.

• SHAPE–Seq: High‐Throughput RNA Structure Analysis

  Citation: 72

  Authors: Stefanie A., Cole, Sharon, Lior, Julius B.

• Selecting, Acquiring, and Using Small Molecule Libraries for High‐Throughput Screening

  Citation: 69

  Authors: Sivaraman, Gerard, Noel, Joseph M., Craig J.