Cold Spring Harbor Protocols

Aims & Scope

Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques.

For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology.

Now that access has moved online.

Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging.

Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.

All protocols are up-to-date and presented in a consistent, easy-to-follow format.

View Aims & Scope

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Metrics & Ranking

Journal Rank

Year	Value
2024	11607

Journal Citation Indicator

Year	Value
2024	270

SJR (SCImago Journal Rank)

Year	Value
2024	0.499

Quartile

Year	Value
2024	Q2

h-index

Year	Value
2024	71

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Abstracting & Indexing

Journal is indexed in leading academic databases, ensuring global visibility and accessibility of our peer-reviewed research.

• Scopus
• Google Scholar
• Medline
• Embase

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Subjects & Keywords

Journal’s research areas, covering key disciplines and specialized sub-topics in Biochemistry, Genetics and Molecular Biology, designed to support cutting-edge academic discovery.

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Most Cited Articles

The Most Cited Articles section features the journal's most impactful research, based on citation counts. These articles have been referenced frequently by other researchers, indicating their significant contribution to their respective fields.

• Illumina Sequencing Library Preparation for Highly Multiplexed Target Capture and Sequencing

  Citation: 1834

  Authors: Matthias, Martin

• Purification of RNA Using TRIzol (TRI Reagent)

  Citation: 1292

  Authors: Donald C., Manuel, Gregory J., Timothy W.

• Hematoxylin and Eosin Staining of Tissue and Cell Sections

  Citation: 1085

  Authors: Andrew H., Kenneth A., Jack, Rolf

• Crystal Violet Assay for Determining Viability of Cultured Cells

  Citation: 673

  Authors: Maria, Peter, Martin

• RNA Sequencing and Analysis

  Citation: 658

  Authors: Kimberly R., Stephen B.

• Analysis of Cell Viability by the MTT Assay

  Citation: 604

  Authors: Priti, Arvindhan, Pradeep D.

• Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections

  Citation: 590

  Authors: Robert D., Claramae H., Robert J.

• DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells

  Citation: 506

  Authors: Lingyun, Gregory E.

• Purification of Nucleic Acids by Extraction with Phenol:Chloroform

  Citation: 495

  Authors: Joseph, David W.

• Bone Marrow-Derived Macrophages (BMM): Isolation and Applications

  Citation: 429

  Authors: Joachim, Bo

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