Protein Expression and Purification

Aims & Scope

Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field.

The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods.

However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.

View Aims & Scope

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Metrics & Ranking

Impact Factor

Year	Value
2025	1.2
2024	1.40

SJR (SCImago Journal Rank)

Year	Value
2024	0.388

Quartile

Year	Value
2024	Q3

h-index

Year	Value
2024	99

Journal Rank

Year	Value
2024	14105

Journal Citation Indicator

Year	Value
2024	604

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Abstracting & Indexing

Journal is indexed in leading academic databases, ensuring global visibility and accessibility of our peer-reviewed research.

• Scopus
• Google Scholar
• Web of Science
• Medline

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Subjects & Keywords

Journal’s research areas, covering key disciplines and specialized sub-topics in Biochemistry, Genetics and Molecular Biology, designed to support cutting-edge academic discovery.

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Most Cited Articles

The Most Cited Articles section features the journal's most impactful research, based on citation counts. These articles have been referenced frequently by other researchers, indicating their significant contribution to their respective fields.

• Protein production by auto-induction in high-density shaking cultures

  Citation: 5239

  Authors: F. William

• Purification of brain tubulin through two cycles of polymerization–depolymerization in a high-molarity buffer

  Citation: 706

  Authors: Mirco, Andrei V.

• Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors

  Citation: 589

  Authors: Peter, Sarah, Zygmunt

• Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

  Citation: 531

  Authors: José, Conni, Gitte E., John

• Immobilized metal ion affinity chromatography

  Citation: 514

  Authors: Jerker

• A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site

  Citation: 484

  Authors: Lucy, Minyi, Lynda, Rosemarie, Frank R., Mark I.

• SUMO fusion technology for difficult-to-express proteins

  Citation: 421

  Authors: Tauseef R., Suzanne C., John P., Michael R.

• Comparison of affinity tags for protein purification

  Citation: 346

  Authors: Jordan J., Joshua L., Heather D., Daniel J., Song

• Practical considerations in refolding proteins from inclusion bodies

  Citation: 341

  Authors: Kouhei, Daisuke, Izumi, Tsutomu

• An overview of enzymatic reagents for the removal of affinity tags

  Citation: 292

  Authors: David S.

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